Abstract

Organoid models have increasingly gained popularity in biological research and screening to recapitulate complexity of real tissues. To model the in vivo human lungs, we have cultured primary human lung epithelial cells under conditions that promote the formation of 3D structures recapitulating the morphological and functional characteristics of the airway. In this poster we describe a high-content imaging method that allows monitoring and visualization of growth and differentiation of lung organoids, 3D reconstitution of the structures, complex analysis of organoid structure, cell morphology and viability, as well as expression of different cell markers. We also demonstrate use of the model for toxicity evaluation of pharmaceutical drugs and other  compounds.