3D cell models representing various tissues were successfully used for studying complex biological effects, tissue architecture, and functionality. 

However, the complexity of 3D models remains a hurdle for wider adoption in research and drug screening.

We describe an automated integrated system that would allow automated monitoring, maintenance, and characterization of growth and differentiation of organoids and stem cells, as well as testing the effects of various compounds. We demonstrated methods for automation of three complex workflows: iPSC culture, 3D lung organoids, and intestinal organoids. The automated, integrated system included an ImageXpress® Confocal HT.ai High-Content Imaging System, automated incubator, Biomek i7 Hybrid workstation, and a robotic device.

The workflow demonstrates the usefulness of automation and advanced high-content imaging for increased throughput and information in organoid assays, critical for compound screening.