Abstract

The JUMP-CP consortium (Joint Undertaking for Morphological Profiling using Cell Painting) is a global collaboration across twelve pharmaceutical and non-profit partners, led by the Broad Institute. The goal of the consortium is to create the world’s largest Cell Painting dataset containing over 140,000 chemical and genetic perturbations. Cell Painting is a high-content imaging assay used to assess the phenotypic changes induced by a perturbation. Morphological profiles can be applied to a variety of applications in drug discovery, including mechanism of action determination, toxicity prediction and matching bioactive compounds to disease states. This valuable dataset will be publicly released on 1st November 2022. The consortium will optimise and share best-practice in image-based profiling, from experimental design to the assay protocol and computational pipelines for image analysis . We present our internal methodology used to fulfil our commitment to the JUMP-CP consortium ahead of final data release.