Abstract

To establish 3D cell cultures in a simple and automation-compatible manner, use the ready-to-use TrueGel3D® HTS Hydrogel Plate! The 96-well polystyrene glass bottom plates contain precast, fully synthetic, functionalized PEG-based hydrogels. These innovative hydrogels contain gradually increasing crosslinking densities throughout the well. Users can proceed directly with seeding cells without any hydrogel preparation or encapsulation steps. After cell seeding, cells will gradually infiltrate the hydrogel and establish a 3D cell culture environment within days. We will present an application example where T cells are co-cultured together with Tumor cells and where we can demonstrate that an activation compound stimulates T cell cytotoxicity specific against the Tumor cells.