Abstract

Different environmental cues such as stress, nutrients or drugs, trigger rapid adaptive responses that allow to maintain cellular homeostasis. One of the fastest cellular responses to the environment is the binding of small molecules to proteins. These molecular interactions produce allosteric effects, which means that they trigger a variation of protein activity as a consequence of a conformational change. Allosteric interactions are thus essential for life and can modulate both the metabolic status of the cells and gene expression. My group focuses on the development of new MS-bases proteomics technologies to study global structural changes in the proteome. In my talk I will describe the applications and latest updates of a proteomic technique called LiP-MS to discover novel protein-drug interactions in microbial and human organisms.