Objective

Epigenetic profiling is critical for understanding the underlying mechanisms involved in cell fate decisions, cellular response to treatment and disease. Unfortunately, no single assay can provide a comprehensive view of the epigenetic state of your cells of interest. Here, we present four different services offered by Active Motif that, when performed in unison, are able to provide a comprehensive understanding of epigenetic determinants that are involved in your model system.  Active Motif’s comprehensive service offering includes: 1) ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing), which interrogates chromatin accessibility changes in your cells or tissues of interest; 2) Mod Spec^TM, which quantifies histone post-translational modifications by mass spectrometry, making it possible to measure global changes of greater than 80 different histone modification states in a single assay; 3) RRBS (Reduced Representation Bisulfite Sequencing) which provides  single base-pair resolution methylation status at a at over 75% of CpG islands and over 50% of promoters; 4) RIME (Rapid Immunoprecipitation Mass Spectrometry of Endogenous Proteins) which can elucidate physically-interacting co-regulators that may be required in establishing the functional specificity of your target protein. Together, these assays will provide you with multi-dimensional mechanistic insight into the factors and pathways involved in the response of your model system.