Objective
High-throughput automated patch clamp (APC) is quickly emerging as the
new gold standard technology for screening compounds against ion channel
targets. The latest APC platforms, such as the Sophion Qube, enable true giga-seal
quality data generation, comparable to conventional patch clamp.
Traditional plate-based screening technologies rarely allowed for
analysis of multiple parameters to detect potential differences in the
mechanism of action of novel compounds. Using multiple data analysis cursors in
the Sophion Analyzer software, it’s possible to gain valuable insight into compound
activities on a particular ion channel target, which may translate into different
results in further in vivo
experiments.
This also increases the value of APC data, as multiple parameters can be
extracted from each well for both data quality control as well as hit detection.
Here we present Qube data for a 10,000 compound pilot screen for a sodium
channel activator project, with analysis for multiple hit parameters,
demonstrating the ability of the assay to detect novel compounds with different
mechanisms of action.
