Discussion

Activity of imidocloprid (18.20% w/v) commercially available was assessed against Hyalomma marginatum in Nili Ravi buffalo (Bubalus bubalus indicus) at Multan, Pakistan. Various concentrations i.e. 0.01% and 0.02% of imidocloprid (18.20% w/v) were prepared. The live ticks were obtained from buffalo(s) with no history of any acaricidal drug prior 60 days and beyond. The tick(s) male and females were identified as Hyalomma marginatum on the basis of pictorial key and morphology and their photograph were preserved and saved for further study. The live tick(s) were immersed in preparation(s) 0.01% and 0.02% in petri-dish(s). Anti-tick activity of imidocloprid against ticks Hyalomma marginatum was observed in 3-5 minutes after application and immersion. There was no any significant difference of anti-tick activity of various concentrations of imidocloprid on Hyalomma marginatum. The treatment was highly effective against the ticks in buffalo.