Helminth glycans at the host-parasite interface

Wed11  Apr09:30am(30 mins)
Where:
Stream 1 - Edward Llwyd 0.26 Biology Main
Session:
Keynote Speaker:
Prof Cornelis Hokke

Authors

C Hokke1
1 Leiden University Medical Center, UK

Discussion

Helminths express an abundance of proteins and lipids with specific and complex glycosylation patterns. Glycomics studies of helminth glycosylation are providing more and more insights into glycan and glycoconjugate expression of different helminth species. Schistosomes are one of the best studied parasitic helminths with respect to structural as well as functional glycomics. In the mammalian host antigenic glycans of larvae, worms and eggs of schistosomes induce specific antibodies to numerous glycan motifs, and glycans initiate or modulate innate immune responses and cellular uptake of glycoconjugates via host lectins. In the intermediate snail host specific glycans of miracidia and sporocysts interact with glycan-binding proteins in the hemolymph determining aspects of snail-schistosome compatibility. To provide a clear map of schistosome glycosylation in support of functional studies of host-parasite glycobiology we applied mass spectrometric (MS) glycomics approaches to determine the expression profiles and structural identity of hundreds of glycans expressed during the schistosome life cycle. Striking shifts and switches in the expression of putative functional glycan motifs during during worm and egg development were identified suggesting various roles of glycans and associated anti-glycan responses during infection. In addition, we have generated a microarray of hundreds of N-, O-, and lipid-glycans covering the entire glycome of S. mansoni. The constructed glycan microarray was used to determine IgG and IgM to each glycan in a number of human and animal infection cohorts. Using clear examples I will discuss how glycan motifs contribute to immunological properties of schistosome antigens, such as the immunomodulatory omega-1 glycoprotein. Also, based on cross-sectional and longitudinal glycan screens of antibodies in sera from natural and experimental schistosome infections, I will discuss the potential of antibodies against schistosome-specific FucĪ±1-2FucĪ±1-3-R glycan motifs in the development of glycan targets for vaccines and diagnostics. Glycomics-driven studies of helminths and helminth infections provide clear contributions to the development of novel therapeutics and control tools.
Schedule

Hosted By
British Society for Parasitology (BSP)
We are science based charitable society.
Event Logo Find us on Facebook Follow us on Twitter

Get the App
Get this event information on your mobile by
going to the appstore or google play and search for 'eventflo'
www.myeventflo.com/2048
Login
Eventflo Home
copyright British Society for Parasitology (BSP), eventflo, Labhoo Ltd 2018