Nanopore sequencing significantly improves genome assembly of the protozoan parasite Trypanosoma cruzi

Mon15  Apr05:57pm(3 mins)
Poster
30
Where:
Renold C16
Miss Florencia Díaz-Viraqué

Authors

F Díaz-Viraqué2; G Iraola1; S Pita2; G Greif2; R Cássia Moreira de Souza3C Robello2
1 Institut Pasteur Montevideo - Laboratorio de Genómica Microbiana, Uruguay;  2 Institut Pasteur Montevideo - Laboratory of Host Pathogen Interactions, Uruguay;  3 Instituto Rene Rachou-FIOCRUZ, Brazil

Discussion

Chagas disease was described by Carlos Chagas, who first identified the parasite Trypanosoma cruzi from a two-year-old girl called Berenice. Many T. cruzi sequencing projects based on short reads have demonstrated that genome assembly and downstream comparative analyses are extremely challenging in this species, given that half of its genome is composed of repetitive sequences. Here, we report de novo assemblies, annotation and comparative analyses of the Berenice strain using a combination of Illumina short reads and MinION long reads. Our work demonstrates that Nanopore sequencing improves T. cruzi assembly contiguity and increases the assembly size in ~16 Mb. Specifically, we found that assembly improvement also refines the completeness of coding regions for both single copy genes and repetitive transposable elements. Beyond its historical and epidemiological importance, Berenice constitutes a fundamental resource since it now represents the best-quality assembly available for TcII, a highly prevalent lineage causing human infections in South America. The availability of Berenice genome expands the known genetic diversity of T. cruzi and facilitates more comprehensive evolutionary inferences. Our work represents the first report of Nanopore technology used to resolve complex protozoan genomes, supporting its subsequent application for improving trypanosomatid and other highly repetitive genomes.

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