DiscussionAlvetex Scaffold 96 & 384 well plates present a simple and reliable alternative to the clonogenic soft agar (TCA) assay for the screening of novel anti-cancer drugs. The technology is compatible with common luminescent/ fluorescence cell health assays reading directly from the plates. In our hands, tumour cells can be cultured for 7-14 days without the need for media changes making the solution very cost effective. The assay was easily automated on a Tecan Freedom EVO 200 robotic platform. The ability to reliably recover RNA and protein from the drug-treated 3D cells from Alvetex Scaffold offers effective analysis of drug mechanism of action using both protein and gene expression techniques.