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From Genes to protein-ligand structures; a work-flow tuned for structural characterization by solution NMR


Structure-based drug design (SBDD) is dependent on the ready availability of 3D structure information, typically derived from X-ray crystallography. However, in many cases this is no successful. NMR is an alternative approach that can provide information on protein-ligand complexes at atomic resolution. In order to support NMR-driven SBDD, ZoBio has established a work flow that allows rapid assessment of soluble protein constructs and conditions that are suitable for executing structure elucidation of protein-ligand complexes by NMR. The typical steps taken in a “gene to protein-ligand structure” project at ZoBio are presented in this poster.

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