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Cytochrome P450 compound profiling


Here we present a high density screening of cytochrome P450 on the Fluentâ„¢ laboratory automation solution. 384-well microplate format enabled maximum information to be gained while minimizing reagent and compound consumption. Throughput was further increased by simultaneous screening of a number of different CYP enzymes or the use of a combined dose-response/single concentration approach. The close correlation of the experimentally calculated and benchmark IC50 values for known enzyme inhibitors shows the excellent quality of the data generated.
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