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Electric Field Stimulation (EFS) of human iPSC-derived neuron using Hamamatsu FDSS/μCELL


Hamamatsu developed a 96-channel electrode array system that is mounted on the
FDSS/μCELL. It adds electric field stimulations (EFS) to all 96 wells in a microplate
simultaneously while fluorescence/luminescence signals are monitored. Using this
instrumental setup, we electrically stimulated human iPSC-derived neurons (iCell®
Neurons, Cellular Dynamics International) to evoke a Ca2+ response, transient
increase of intracellular Ca2+ concentration, which was measured with calciumsensitive
fluorescent dyes.
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