Programme : Back to Dr Christoph Sachse
Poster
44

Characterisation of β-catenin complexes by Chromobody-based intracellular co-immunoprecipatation and DigiWest protein profiling

Discussion

R Haeussler, B Traenkle, F Treindl, M Templin, U Rothbauer

Natural and Medical Sciences Institute (NMI) at the University of Tübingen; University of Tübingen; NMI TT Pharmaservices Berlin, Germany

β-catenin is the central regulatory protein in the canonical Wnt-pathway, which controls essential physiological processes such as cell proliferation, differentiation and apoptosis; aberrant mutational activation of central players of this signaling cascade are found in many cancer cells. The function of β-catenin is controlled by its localization, stability and integration into different protein complexes. We look directly at β-catenin complexes using a novel Chromobody-based intracellular co-immunoprecipitation approach in combination with the multiplex protein profiling platform DigiWest.
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