Programme : Back to Mr François Degorce
Poster
33

Universal HTRF® phospho-protein platform : from 2D, 3D, primary cells to patient derived tumor cells

Discussion

The monitoring of key signaling nodes, such as the MAP-Kinase ERK or PI3 Kinase/AKT, became a commonly used approach in investigations on signaling networks. These proteins represent either cellular readouts in a cascade of signaling events or are considered as the primary targets for compounds development. Therefore, the quantification of their phosphorylation status is of a great importance. For many years, the western-blot was the most popular technique to detect protein phosphorylation. More recently, assays based on the HTRF method, an advanced fluorescence-based technology, were developed to facilitate the research on phospho-proteins and more generally on signaling pathways.

Here we provide valuable results obtained with different HTRF assay on a large panel of cellular models, such as well-established cell lines grown in 2D or 3D, normal primary cells such as PBMC, or primary tumor and metastatsis patient-derived cancer. All together, these data show that the HTRF phospho-proteins platform can be easily implemented to investigate a large diversity of sample types, including primary cells.

Cruz T (1), Vallaghé J (2), Chouvet C (2), Morcillo V (2), Douzon S (2), Costy C (2), Oberleitner S (2), Trinquet E (2), Pannequin J (1), Charrier-Savournin F (2)

(1)Institut de génomique Fonctionnelle, CNRS UM5203, INSERM U661, Montpellier, France
(2)Cisbio Bioassays, Codolet, France
Programme
Add to favourites

Hosted By
ELRIG
The European Laboratory Research & Innovation Group Our Vision : To provide outstanding, leading edge knowledge to the life sciences community on an open access basis
Event Logo Find us on Facebook Follow us on Twitter

Get the App
Get this event information on your mobile by
going to the appstore or google play and search for 'elrig'
iPhone App
Android App
www.myeventflo.com/1791
Login
Eventflo Home
copyright ELRIG, eventflo, Labhoo Ltd 2018